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KMID : 0380020050200030244
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 3 p.244 ~ p.249
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Separation Characteristics of Ibuprofen in Kromasil HPLC Column
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Park Joon-Sub
Kim Byung-Lip
Yoon Tae-Ho
Kim In-Ho
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Abstract
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Chiral separation of racemic ibuprofen was achieved on a Kromasil KR100-5CHI-TBB column. Some chromatographic parameters (resolution, number of theoretical plates, HETP, capacity factor) are calculated under different separation conditions such as change of mobile phase compositions (hexane / t-BME : 85 / 15, 75 / 15, 65 / 35, 55 /45) as well as acetic acid concentrations for adjusting pH (0.1 to 1 ). Flow rate versus number of theoretical plates and HETP were compared to evaluate column efficiency. To determine the adsorption isotherms, PIM (Pulsed Input Method) was carried out. At concentrations of racemic ibuprofen between 0.1 and 0.3 mg/ml, S- and R-ibuprofen have the same retention time of 4.48 and 5.81 min. Ibuprofen isotherms show a linear form under concentrations of 0.3 mg/ml with eluent (hexane / t-BME = 55 / 45).
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KEYWORD
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Ibuprofen, chiral separation, HPLC, PIM
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